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Modeling of phosphate pools dynamics during the growth of Chlorella vulgaris with different availability of phosphorus in the mediumтезисы доклада
- Авторы: Tatiana Plyusnina, Polina Fursova, Sergey Khruschev, Alexei Solovchenko
- Сборник: 10th International Conference “Photosynthesis and Hydrogen Energy Research for Sustainability-2019", June 23 – 28, 2019, Saint Petersburg, Russia, Abstracts, ISBN 978-5-4386-1743-3
- Тезисы
- Год издания: 2019
- Место издания: Komarov Botanical Institute of the Russian Academy of Sciences St. Petersburg
- Первая страница: 163
- Последняя страница: 163
- Аннотация: Microalgae cells are able to accumulate phosphorus in the form of polyphosphates, which are involved in cell energy metabolism. Understanding the mechanisms of regulation and the ability to control the accumulation of polyphosphate pools can be used in solving various biotechnological problems. A hybrid model of phosphate pools dynamics during the growth of Chlorella vulgaris cells under different media conditions is proposed. The model links different physiological levels of cellular processes and consists of two blocks. The first block is a system of 5 differential equations describing the phosphorus uptake from the environment, the inclusion of inorganic phosphate into biomolecules, the formation and growth of a polyphosphates pool, biomass increase during culture growth. The change in intracellular concentrations of phosphorus compounds is determined by the minimum cell quota for the phosphorus content in the inorganic form and the maximum in the form of biomacromolecules. For parameterization of the model, experimental data on the growth of the culture of Chlorella vulgaris were used. The second block of the model is based on stoichiometric equations of the central metabolic pathways, including glycolysis, the citric acid cycle, respiratory and photosynthetic electron transport chains. The reaction scheme takes into account ATP synthesis and consumption, the binding and release of inorganic phosphorus. A redistribution of the metabolic fluxes for the different availability of inorganic phosphorus in medium was calculated. The model allowed us to detect the growth stage of the culture, at which the maximum accumulation of polyphosphates occurs.
- Добавил в систему: Хрущев Сергей Сергеевич