"SprI/SprR Quorum Sensing System of Serratia proteamaculans 94"

Zaitseva, Y.; Koksharova, O.; Lipasova, V.; Plyuta, V.; V, D.I.; Chernin, L.; Khmel, I.A.

Авторы: Yulia Zaitseva, Olga Koksharova, Valentina Lipasova, Vladimir Plyuta, Demidyuk Ilya V., Leonid Chernin and Inessa A.Khmel
Журнал: BioMed Research International
Том: 2019
Год издания: 2019
Первая страница: 3865780
DOI: 10.1155/2019/3865780
Аннотация: In this study, we investigated the quorum sensing (QS) regulatory system of the psychrotrophic strain Serratia proteamaculans 94isolated from spoiled refrigerated meat. -e strain produced several N-acyl-L-homoserine-lactone (AHL) QS signal molecules,with N-(3-oxo-hexanoyl)-L-homoserine lactone and N-(3-hydroxy-hexanoyl)-L-homoserine lactone as two main types. -e sprIand sprR genes encoding an AHL synthase and a receptor regulatory protein, respectively, were cloned and sequenced. Analysis oftheir nucleotide sequence showed that these genes were transcribed convergently and that their reading frames partly overlappedby 23 bp in the terminal regions. -e genes were highly similar to the luxI/luxR-type QS genes of other Gram-negative bacteria. Anspr-box (analog of the lux-box) was identified upstream of the sprR gene and found to be overlapped with the sequence of − 10sequence site in the promoter region of this gene. Inactivation of the sprI gene led to the absence of AHL synthesis, chitinolyticactivity, and swimming motility; decrease of extracellular proteolytic activity; affected the cellular fatty acid composition; and reducedsuppression of the fungal plant pathogen mycelium growth by volatile compounds emitted by strain S. proteamaculans 94. -e dataobtained demonstrated the important role of the QS system in the regulation of cellular processes in S. proteamaculans 94.
Добавил в систему: Кокшарова Ольга Алексеевна

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"SprI/SprR Quorum Sensing System of Serratia proteamaculans 94"статья

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