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Final checkpoint in the drug-promoted and poliovirus-promoted apoptosis is under post-translational control by growth factorsстатья
Информация о цитировании статьи получена из
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Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Tolskaya E.A., Romanova L.I., Kolesnikova M.S., Ivannikova T.A., Agol V.I.
- Журнал: Journal of Cellular Biochemistry
- Том: 63
- Номер: 4
- Год издания: 1996
- Издательство: John Wiley & Sons Inc.
- Местоположение издательства: United States
- Первая страница: 422
- Последняя страница: 431
- DOI: 10.1002/(SICI)1097-4644(19961215)63:4<422::AID-JCB4>3.0.CO;2-V
- Аннотация: The treatment of HeLa subline (HeLa-B) cells with cycloheximide or Actinomycin D resulted in a rapid (approximately 1.5 h and approximately 2.5 h, respectively) development of morphological and biochemical signs of apoptosis. The addition of fetal bovine serum to the cycloheximide-treated or Actinomycin D-treated cells suppressed the apoptotic reaction, as evidenced by the postponement of the DNA fragmentation for at least 9 and 5 h, respectively. A similar suppressive effect was observed upon the serum addition to cells undergoing abortive infection with poliovirus, which died of apoptosis in the absence of the serum. The serum appeared to exert its anti-apoptotic effect without any appreciable lag and even immediately blocked further progress of ongoing DNA fragmentation. The epidermal growth factor also suppressed, although less efficiently and more transiently, the apoptotic reaction promoted by the metabolic inhibitors. It is concluded that growth factors may affect, without modulating either transcription or translation, the balance of pro-apoptotic and anti-apoptotic activities at a final checkpoint, just preceding the irreversible effector step of apoptosis.
- Добавил в систему: Агол Вадим Израилевич