Properties of hexahistidine-tagged organophosphate hydrolaseстатья
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Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
Аннотация:The catalytic properties of organophosphate hydrolase (OPH) containing a hexahistidine tag His(6) (His(6)-OPH) and purified to 98% homogeneity were investigated. The pH optimum of enzymatic activity and isoelectric point of His(6)-OPH, which were shown to be 10.5 and 8.5, respectively, are shifted to the alkaline range as compared to the same parameters of the native OPH. The recombinant enzyme possessed improved catalytic activity towards S-containing substrates: the catalytic efficiency of methylparathion hydrolysis by His(6)-OPH is 4.2(.)10(6) M(-1.)sec(-1), whereas by native OPH it is 3.5(.)10(5) M(-1.)sec(-1).