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The effect of long-term preservation of bacterial cells immobilized in poly(vinyl alcohol) cryogel on their viability and biosynthesis of target metabolitesстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Efremenko E.N., Tatarinova N.Y.
- Журнал: Microbiology
- Том: 76
- Номер: 3
- Год издания: 2007
- Издательство: Pleiades Publishing, Ltd
- Местоположение издательства: Road Town, United Kingdom
- Первая страница: 336
- Последняя страница: 341
- DOI: 10.1134/S0026261707030113
- Аннотация: The effect of cell storage at -18 degrees C for 18-24 months on reproductive capacity was investigated for various microorganisms (gram-positive and gram-negative bacteria, yeasts, and filamentous fungi) immobilized in poly(vinyl alcohol) cryogel. To examine the viability of immobilized cells after defrosting, the bioluminescent method of intracellular ATP determination was used. A high level of metabolic activity of immobilized cells after various periods of storage was recorded for Streptomyces anulatus, Rhizopus oryzae, and Escherichia coli, which are producers of the antibiotic aurantin, L(+)-lactic acid, and the recombinant enzyme organophosphate hydrolase, respectively. It was shown that the initial concentration of immobilized cells in cryogel granules plays an important role in the survival of Str. anulatus and Pseudomonas putida after 1.5 years of storage. It was found that, after slow defrosting in the storage medium at 5 degrees C for 18 h of immobilized cells of the yeast Saccharomyces cerevisiae that had been stored for nine months, the number of reproductive cells increased due to the formation of ascospores
- Добавил в систему: Ефременко Елена Николаевна