|
ИСТИНА |
Войти в систему Регистрация |
ФНКЦ РР |
||
Rotenone-sensitive oxidation of NADH and F0F1-ATPase activity in a homogenate ofrat skeletal muscles during thermal adaptationстатья
Дата последнего поиска статьи во внешних источниках: 28 мая 2015 г.
- Авторы: Ritov VB, Gorbacheva LR, Tverdislova IL, Leĭkin IuN, Bazhenov IuI
- Журнал: Biochemistry (Moscow)
- Том: 58
- Номер: 11
- Год издания: 1993
- Издательство: Pleiades Publishing, Ltd
- Местоположение издательства: Road Town, United Kingdom
- Первая страница: 1779
- Последняя страница: 1787
- Аннотация: A method has been developed for measuring the rates of rotenone-sensitive oxidation of NADH and oligomycin-sensitive hydrolysis of ATP in rat skeletal muscle homogenates. The method is based on the use of alamethicin which increases the permeability of the inner mitochondrial membrane for NADH and ATP. It has been shown that prolonged cold adaptation of rats (4 weeks, 4 degrees) does not change the activity of rotenone-sensitive NADH-oxidase in rat skeletal muscle homogenates which is equal to 12.4 +/- 4.4 nmol NADH/min/mg protein, but increases threefold that of F0F1-ATPase--from 31.8 +/- 7.4 up to 93.1 +/- 14.3 nmol P(i)/min/mg protein. It is suggested that prolonged cold adaptation induces structural-and-functional changes in the H(+)-ATP-synthetase complex of skeletal muscle mitochondria.
- Добавил в систему: Горбачева Любовь Руфэльевна