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Fluorescence Polarization Immunoassay for Highly Efficient Detection of Imidaclothiz in Agricultural Samplesстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 24 октября 2016 г.
- Авторы: Ming Ma, Chen Mo, Feng Lu, You Hongjie, Yang Rui, Boroduleva Anna, Hua Xiude, Eremin Sergei A., Wang Minghua
- Журнал: Food Analytical Methods
- Том: 9
- Номер: 9
- Год издания: 2016
- Издательство: Springer Pub. Co.,
- Местоположение издательства: United States
- Первая страница: 2471
- Последняя страница: 2478
- DOI: 10.1007/s12161-016-0434-5
- Аннотация: A homogeneous fluorescence polarization immunoassay (FPIA) based on a monoclonal antibody for the detection of imidaclothiz was developed. Two fluorescein-labeled imidaclothiz tracers containing two different bridge lengths were synthesized and purified. Under optimal conditions, the 4-aminofluorescein-labeled imidaclothiz conjugate (AMF-labeled imidaclothiz), which contains a shorter bridge length, showed a higher sensitivity in the FPIA for detecting imidaclothiz, and the full analysis was achieved in less than 11 min. The IC50 and limit of detection (LOD, IC10) were 87.94 ± 10.18 and 0.57 ± 0.16 μg/L, respectively. The spiked recoveries were 83 to 117 % measured in tomato, pear, rice, apple, cucumber, cabbage, and paddy water, with RSDs of 5 to 12 %. Furthermore, the results of FPIA for the authentic samples correlated well with those acquired by HPLC. Overall, the developed FPIA provided a simple, rapid, sensitive, and accurate method that was used for the quantitative detection of imidaclothiz in agricultural samples.
- Добавил в систему: Еремин Сергей Александрович
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | 16_fam9_2471.pdf | 16_fam9_2471.pdf | 652,0 КБ | 4 октября 2016 [Sergei_A_EREMIN] |