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Characterization of hepatitis C virus structural proteins and HCV-like particles produced in recombinant baculovirus infected insect cellsстатья
Информация о цитировании статьи получена из
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 12 января 2016 г.
- Авторы: Belzhelarskaia S.N., Koroleva N.N., Popenko V.V., Drutsa V.L., Orlova O.V., Rubtsov P.M., Kochetkov S.N.
- Журнал: Molecular Biology
- Том: 44
- Номер: 1
- Год издания: 2010
- Издательство: Maik Nauka/Interperiodica Publishing
- Местоположение издательства: Russian Federation
- Первая страница: 107
- Последняя страница: 119
- DOI: 10.1134/S0026893310010139
- Аннотация: Three proteins, namely: "core" protein C and glycoproteins E1 and E2, are main structural proteins forming a hepatitis C vius (HCV) virion. The virus structure and assembly, a role of the structural proteins in virion morphogenesis remain unknown because of the lack of an efficient culture system for HCV to be grown in vitro. Using recombinant baculoviruses expressing HCV structural protein genes in insect cells the specific structural proteins at the level of 25-35% relative to a common cell protein content, heterodimers of the glcoproteins, and HCV-like particles have been obtained. It has been demonstrated that recombinant proteins C, E1, and E2 go through the posttranslation modification, the glycoproteins form the non-covalent heterodimer, and HCV-like particles are located in endoplasmatic reticulum membrains of infected cells. An ability of the expressed proteins for forming E1E2 dimers and HCV-like particles was used for studying the role of E1 protein glcosylation upon expression and processing of the glycoproteins.
- Добавил в систему: Друца Валерий Львович