Formation of connections between cultured identified neurons from the pleural ganglion of the pteropod mollusk clione-limacinaстатья
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Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
Аннотация:A cluster of electrically interconnected neurosecretory cells (the 'white cells') involved in the control of reproductive behavior was identified in the right pleural ganglion of the marine mollusc, Clione limacina. Pleural ganglia also contain large neurons (PL1 and PL2) having no connections with each other and with the white cells. Most isolated white cells put into the simple unconditioned medium (50% L-15) adhered to the bottom of uncoated dishes and demonstrated neurite outgrowth for 7-10 days. If growing processes overlapped, the white cells formed electrical connections with each other, but they formed no connections with the PL1 and PL2 neurons. It is concluded that in the case which was under study cellular intrinsic properties were sufficient for the formation of 'correct' connections between neurones.