Conversion of Soybean Phytosterol into Androsta-4,9(11)-diene-3,17-dioneстатья
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Дата последнего поиска статьи во внешних источниках: 19 августа 2020 г.
Аннотация:In this study, we developed a method for the production of androsta-9(11)-diene-3,17-dione(Δ9(11)-AD), which is a combination of phytosterol side-chain microbial oxidation with simultaneous9α-hydroxylation and subsequent chemical regio-selective dehydration of 9α-hydroxy-3,17-diketo-intermediatewithout isolation and purification. Phytosterol was converted into 9α-hydroxyandrost-4-ene-3,17-dione (9-OH-AD) with the use of the wild-type Mycobacterium sp. VKM Ac-1817D strain. The product wasextracted from the culture medium with the use of an organic solvent and dehydrated in the extract with mineralacid. The resulting Δ9(11)-AD was purified with the selective crystallization method. Minor products wereisolated and identified. It has been shown that this strain is capable of sterol transformation with the formationof methyl ester of 9α-hydroxypregn-4-ene-3-one-20-carboxylic acid. Our approach makes it possible tosimplify the flow chart for production of the target compound. It not only eliminates 9-OH-AD loss but alsominimizes the amount of production waste.