Аннотация:In this work we suggest the method for indication the Methylorosula species in natural samples. The full description of Methylorosula polaris has already been published. The method based on it helps to distinguish Methylorosula polaris between other ecologically and taxonomically similar species in several simple stages. The method requires visual examination of Petri dishes, light microscopy and substrate growth testing. It is possible to modify this method in order to detect other methylotrophic bacteria.