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Immobilized lysozyme for removal of bacteriallipopolysaccharide (endotoxin) from biological fluidsтезисы доклада
Информация о цитировании статьи получена из
Web of Science
Дата последнего поиска статьи во внешних источниках: 13 января 2021 г.
- Авторы: Matolygina D.A., Ovchinnikova E.D., Adamova I.Y., Dmitrieva O.A., Nuzhdina A.V., Karelina N.V., Nelyub V.A., Pokrovsky N.S., Eremeev N.L., Levashov P.A.
- Сборник: FEBS Open Bio
- Серия: Suppl. 1
- Том: 9
- Тезисы
- Год издания: 2019
- Место издания: John Wiley & Sons Inc United States
- Первая страница: 209
- Последняя страница: 209
- Номер статьи: P-20-003
- DOI: 10.1002/2211-5463.12675
- Аннотация: In connection with the emergence of new antibiotic resistantstrains of bacteria in the world, the problem of treatment of sep-sis has become more acute. One of the key tasks in the treatmentof severe sepsis is the ability to remove bacterial endotoxins fromthe patient’s bloodstream. A high concentration of bacterialendotoxins in the human blood disrupts the normal functioningof the immune system and dramatically worsens the patient’s condition. The removal of endotoxins is carried out in extracor-poreal therapy procedures using sorbents. Thus, the developmentof new effective sorbents for the removal of endotoxin is animportant task. Lysozyme is able to bind with the components ofthe surface of a bacterial cell, so we have suggested that a lyso-zyme based sorbent can potentially be able to bind bacteriallipopolysaccharides. The sorbent made by us contains 14–15 mgof a covalently immobilized lysozyme per 1 g of an insolublepolysaccharide matrix (cross-linked agarose). This sorbent showshigh efficiency of endotoxin binding from solution: 50 mL of sor-bent can remove up to 88% Escherichia coli endotoxin or 84%Pseudomonas aeruginosa endotoxin from a 500 mL solution withan endotoxin concentration of 500 EU / mL (50 ng / mL).
- Добавил в систему: Матолыгина Дарья Андреевна