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Simultaneous Quantification of Plasma Catecholamines and Metanephrines by LC‑MS/MSстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 2 декабря 2020 г.
- Авторы: Dikunets M., Dudko G., Glagovsky P., Mamedov I.
- Журнал: Journal of the Brazilian Chemical Society
- Том: 31
- Номер: 7
- Год издания: 2019
- Издательство: Sociedade Braslleira de Quirnica
- Местоположение издательства: Brazil
- Первая страница: 1467
- Последняя страница: 1474
- DOI: 10.21577/0103-5053.20200033
- Аннотация: The quantitation of the low levels of catecholamines and metanephrines in biological fluids is important for clinical screening of pheochromocytoma/paraganlioma and diagnosis of overtraining syndrome in athletes. We introduce a novel, accurate and sensitive liquid chromatography-tandem mass spectrometry method for simultaneous quantitation of these biogenic amines in human plasma. Simple protein precipitation combined with rapid liquid-liquid extraction with ethyl acetate and 2-aminoethyl diphenylborinate as the complexing reagent allow us to quantify catecholamines and metanephrines over broad concentration ranges. Target analytes and their respective deuterated internal standards were monitored in positive electrospray ionization mode by multiple reaction monitoring. Method performance was validated for linearity, lower limit of quantitation, limit of detection, intra-day and inter-assay precision and carry-over. The assay was linear within analytical range 25–1 000 pg/mL for epinephrine, 30–2 500 pg/mL for norepinephrine, 15–1 000 pg/mL for dopamine, 25–2 000 pg/mL for metanephrine and 50–10 000 pg/mL for normetanephrine, with lower limits of quantification of 15, 20, 10, 15 and 30 pg/mL, respectively The intra- and inter-day precisions for all compounds ranged from 0.6 to 2.4% and from 2.3 to 7.6%, respectively. The efficiency of novel method was confirmed by assaying external quality control samples and perfect results with consistency to the target ranges were obtained.
- Добавил в систему: Мамедов Ильгар Салехович