Аннотация:Mitochondrial mutational signature is very conserved and low deviations between species have been associated with longevity. By reconstructing species-specific mtDNA mutational spectrum for ray-finned fishes (Actinopterygii), we observed that temperature is a strong additional factor shaping the mtDNA mutational spectrum in ectotherms. The analysis of mammalian endotherms, with a special focus on species with temporarily or permanently low metabolic rates (hibernators, daily torpors, naked mole rat, etc.), confirmed the temperature effect, suggesting that two main factors shape between-species variation in mitochondrial mutational spectra: longevity and temperature.DOI: https://doi.org/10.1101/2020.07.25.221184