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Nanozymes ‘Artificial Peroxidase’ – Enzymes Oxidases Mixtures for Single‐Step Fabrication of Advanced Electrochemical Biosensorsстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 9 апреля 2021 г.
- Авторы: Komkova Maria A., Andreeva Ksenia D., Zarochintsev Alexander A., Karyakin Arkady A.
- Журнал: ChemElectroChem
- Год издания: 2021
- Издательство: Wiley-VCH Verlag
- Местоположение издательства: Weinheim, Germany, Germany
- Номер статьи: celc.202100275
- DOI: 10.1002/celc.202100275
- Аннотация: We report on a single‐step fabrication of advanced electrochemical biosensors upon drop‐casting of ‘artificial peroxidase’−oxidase containing mixtures. Compared to bienzyme biosensors we substitute peroxidase with more active and stable nanozymes based on catalytically synthesized Prussian Blue. Electroactivity of nanozymes in the sensing membrane was facilitated with carbon black nanoparticles, which resulted in record sensitivity of the H 2 O 2 transducer exceeding 1.1 A M –1 ·cm –2 . Both glucose and lactate oxidases were co‐immobilized with nanozymes in alkoxysilane or perfluorosulfonated ionomer membranes. The proposed drop‐casting approach results in biosensors advantageous over those conventionally produced upon layer‐by‐layer immobilization in terms of i) almost an order of magnitude increased sensitivity, ii) unsurpassed biosensor‐to‐transducer sensitivity ratio reaching the value of 0.3, and iii) three times extended operational lifetime.
- Добавил в систему: Комкова Мария Андреевна