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Low cetyltrimethylammonium bromide concentrations induce reversible amorphous aggregation of tobacco mosaic virus and its coat protein at room temperatureстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Panyukov Y.V., Nemykh M.A., Rafikova E.R., Kurganov B.I., Yaguzhinsky L.S., Arutyunyan A.M., Drachev V.A., Dobrov E.N.
- Журнал: International Journal of Biochemistry and Cell Biology
- Том: 38
- Номер: 4
- Год издания: 2006
- Издательство: Pergamon Press Ltd.
- Местоположение издательства: United Kingdom
- Первая страница: 533
- Последняя страница: 543
- DOI: 10.1016/j.biocel.2005.10.014
- Аннотация: Ordered and amorphous protein aggregation causes numerous diseases. Tobacco mosaic virus coat protein for many decades serves as the classical model of ordered protein aggregation ("polymerization"). It was also found to be highly prone to heat-induced amorphous aggregation and the rate of this aggregation could be easily manipulated by changes in solution ionic strength and temperature. Here, we report that rapid amorphous aggregation of this protein call be induced at 25 degrees C in phosphate buffer by low micromolar (start at about 15 mu M) concentrations of cationic surfactant cetyltrimethylammonium bromide. At equilibrium four surfactant molecules bound to the protein subunit. As judged by circular dichroism and fluorescence spectroscopy data, the coat protein molecules retained their native structure upon the cetyltrimethylammonium bromide induced aggregation. No aggregation was observed at the higher surfactant concentrations (above 300 mu M). Micromolar concentrations of anionic surfactant sodium dodecylsulfate rapidly reversed the cetyltrimethylammonium bromide induced aggregation of the coat protein due to formation of mixed surfactant-surfactant micelles. Cetyltrimethylammonium bromide (100-300 mu M) also induced the reversible intact tobacco mosaic virus virion aggregation. The possible liability to the cetyltrimethylamnionium bromide induced amorphous aggregation of other ordered aggregate-producing proteins has been discussed. (c) 2005 Elsevier Ltd. All rights reserved.
- Добавил в систему: Добров Евгений Николаевич