Sensitive Hydrogen Peroxide Biosensor based on Tobacco Peroxidase Immobilized on p‐Phenylenediamine Diazonium Grafted CNTs Preventing Fenton‐like Inactivation at Negative Potential

Ciogli, L.; Zumpano, R.; Poloznikov, A.A.; Hushpulian, D.M.; Tishkov, V.I.; Andreu, R.; Gorton, L.; Mazzei, F.; Favero, G.; Bollella, P.

Авторы: Ciogli L., Zumpano R., Poloznikov A.A., Hushpulian D.M., Tishkov V.I., Andreu R., Gorton L., Mazzei F., Favero G., Bollella P.
Журнал: ChemElectroChem
Год издания: 2021
Издательство: Wiley-VCH Verlag
Местоположение издательства: Weinheim, Germany, Germany
Номер статьи: celc.202100341
DOI: 10.1002/celc.202100341
Аннотация: Herein, we present a novel electrode platform for H2O2 detection based on the immobilization of recombinant Tobacco Peroxidase (r-TOP) onto graphite electrodes (G) modified with p-phenylenediamine (p-PD) diazonium cation grafted multi-walled carbon nanotubes (MWCNTs). The employment of both p-phenylenediamine moieties and covalent cross-linking by using glutaraldehyde allowed to enhance the sensitivity, stability and selectivity toward H2O2 detection, as well as preventing the enzyme inactivation due to the electro-Fenton reaction. These results are mainly ascribed to a beneficial enzyme immobilization leading to a correct orientation achieved through cross-linking the enzyme in combination with electrostatic interaction between the uncoupled -NH2 groups (mainly uncharged at pH 7, considering a pKa of 4.6) available on the electrode surface and the enzyme. In particular, the electrode based on the r-TOP/p-PD/MWCNTs/G platform showed a lower limit of detection of 1.8 µM H2O2, a extended linear range between 6 and 900 µM H2O2, as well as a significant increase in sensitivity (63.1 ± 0.1 µA mM-1 cm-2) compared with previous work based on TOP. The developed enzyme electrode showed a great stability, losing only 17.5% of its response along 30 days. Finally, the r-TOP/p-PD/MWCNTs/G electrode was tested as a screening analytical method for the detection of H2O2 in food samples.
Добавил в систему: Тишков Владимир Иванович

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Sensitive Hydrogen Peroxide Biosensor based on Tobacco Peroxidase Immobilized on p‐Phenylenediamine Diazonium Grafted CNTs Preventing Fenton‐like Inactivation at Negative Potentialстатья

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