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Quantitative analysis of palladacycle-tagged PLGA nanoparticles biodistribution in rat organs by means of atomic absorption spectrometry and inductively coupled plasma mass spectrometryстатья
Статья опубликована в высокорейтинговом журнале
Статья опубликована в журнале из списка Web of Science и/или Scopus- Авторы: Ermolenko Yulia, Gorunova Olga N., Dunina Valery V., Petrenko Dmitriy B., Novikova Nadejda G., Alekseeva Anna I., Osipova Nadezhda, Kochetkov Konstantin A., Morozov Alexander, Gelperina Svetlana
- Журнал: Journal of Analytical Atomic Spectrometry
- Том: 36
- Номер: JA-ART-05-2021-000162
- Год издания: 2021
- Издательство: Royal Society of Chemistry
- Местоположение издательства: United Kingdom
- DOI: 10.1039/D1JA00260K
- Аннотация: In the present study, the biodistribution of PLGA nanoparticles labelled with Pd in the form of a dimeric cyclopalladated derivative of N,N-dimethyldiphenylmethylamine (PLGA/Pd NPs) was investigated in rats using graphite furnace atomic absorption spectrometry (GFAAS) and inductively coupled plasma mass spectrometry (ICP-MS). Sample preparation conditions were selected for complete degradation (microwave digestion) of biological samples and Pd dissolution for further study by the GFAAS and ICP-MS techniques. The detection limits (LODs) were 0.7 ng mL−1 (GFAAS) and 0.05 ng mL−1 (ICP-MS). The PLGA/Pd NPs obtained by ultrasonication had an average size of ∼189.0 nm; the Pd content was 18.2 μg mg−1 PLGA. The Pd label was stably retained in the palladacycle-loaded PLGA nanoparticles with only 0.7% of Pd released within 4 h of incubation in the model medium (PBS, 37 °C). The Pd content in rat organs was evaluated 2 h post intravenous administration of the PLGA/Pd NPs after complete degradation of biological samples and Pd dissolution for further analysis by the GFAAS and ICP-MS techniques. The biodistribution profile of the Pd characterized by typical for the NP accumulation in the RES organs (liver: 70% of the dose, spleen: 26% of the dose) with minor uptake (<1%) in the lungs, kidneys, brain, and heart suggests that poorly soluble cyclopalladated complexes may serve as suitable labels for evaluation of the NP biodistribution.
- Добавил в систему: Дунина Валерия Владимировна
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | Полный текст | JAAS-36-2423.pdf | 577,7 КБ | 23 декабря 2021 [VDUNINA] | |
| 2. | JAAS-expert.jpg | JAAS-expert.jpg | 1,3 МБ | 23 декабря 2021 [GorunovaON] |