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Antigen‐Specific Stimulation and Expansion of CAR‐T Cells Using Membrane Vesicles as Target Cell Surrogatesстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 14 октября 2021 г.
- Авторы: Ukrainskaya Valeria, Rubtsov Yuri, Pershin Dmitry, Podoplelova Nadezhda, Terekhov Stanislav, Yaroshevich Igor, Sokolova Anstasiia, Bagrov Dmitry, Kulakovskaya Elena, Shipunova Viktoria, Deyev Sergey, Ziganshin Rustam, Chernov Aleksandr, Telegin Georgii, Maksimov Eugene, Markov Oleg, Oshchepkova Anastasiya, Zenkova Marina, Xie Jia, Zhang Hongkai, Gabibov Alexander, Maschan Michael, Stepanov Alexey, Lerner Richard
- Журнал: Small
- Год издания: 2021
- Издательство: Wiley - V C H Verlag GmbbH & Co.
- Местоположение издательства: Germany
- Номер статьи: 2102643
- DOI: 10.1002/smll.202102643
- Аннотация: Development of CAR-T therapy led to immediate success in the treatment of B cell leukemia. Manufacturing of therapy-competent functional CAR-T cells needs robust protocols for ex vivo/in vitro expansion of modified T-cells. This step is challenging, especially if non-viral low-efficiency delivery protocols are used to generate CAR-T cells. Modern protocols for CAR-T cell expansion are imperfect since non-specific stimulation results in rapid outgrowth of CAR-negative T cells, and removal of feeder cells from mixed cultures necessitates additional purification steps. To develop a specific and improved protocol for CAR-T cell expansion, cell-derived membrane vesicles are taken advantage of, and the simple structural demands of the CAR-antigen interaction. This novel approach is to make antigenic microcytospheres from common cell lines stably expressing surface-bound CAR antigens, and then use them for stimulation and expansion of CAR-T cells. The data presented in this article clearly demonstrate that this protocol produced antigen-specific vesicles with the capacity to induce stronger stimulation, proliferation, and functional activity of CAR-T cells than is possible with existing protocols. It is predicted that this new methodology will significantly advance the ability to obtain improved populations of functional CAR-T cells for therapy.
- Добавил в систему: Максимов Евгений Георгиевич