Effect of G-quadruplexes on DNA mismatch repair pathway

Kubareva, Е.; Pavlova, A.; Monakhova, M.; Zvereva, M.; Dolinnaya, N.

Авторы: Kubareva Е., Pavlova A., Monakhova M., Zvereva M., Dolinnaya N.
Сборник: Encyclopedia
Серия: Biochemistry & Molecular Biology
Глава в коллективной монографии
Год издания: 2021
Издательство: MDPI
Местоположение издательства: Basel, Switzerland
Номер статьи: 15927
Аннотация: https://encyclopedia.pub/15927: DNA G-quadruplexes (G4s) are known to be an integral part of the complex regulatory systems in both normal and pathological cells. Here we studied the effect of G4s on the DNA mismatch repair pathway. To assess the role of the distance between G4 and DNA mismatch on the functioning of the key mismatch repair protein, MutS, from E. coli on G4-containing substrates, a set of DNA duplexes with an embedded intramolecular parallel G4 structure and a monomethylated recognition site for the MutH endonuclease was prepared; the distance between the mismatched G/T pair and the G4 structure varied from 18 to 3 bp. It has been shown that this non-B form structure is not perceived by the E. coli system as damage that needs to be repaired; at the same time, it does not prevent activation of DNA mismatch repair, even when the G4 structure and G/T pair are at the distance of 3 bp. Thus, the preferential binding of MutS to G4 does not correlate with DNA mismatch repair activity, suggesting an unexpected role of these DNA-protein interactions in genome maintenance.
Добавил в систему: Кубарева Елена Александровна

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