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Stromal and Tumor Glioma-Derived Cells with Similar Characteristics have Differences in α-Smooth Muscle Actin Expression and LocalizationI. Journal of Oncology Research Vol 3, No 2 (2021)статья
- Авторы: Gin, Chistyakova I., Zenin V., Koshkin S., Musorina A., Lahina Y., Timin G., Pospelov V., Prikhodko S., Petukhov A., Tolkunova E.
- Журнал: Journal of Oncology Research
- Том: 3
- Номер: 2
- Год издания: 2021
- Издательство: Bilingual Publishing Co.
- Местоположение издательства: Singapore
- Первая страница: 8
- Последняя страница: 21
- Аннотация: Gliomas are solid brain tumors composed of tumor cells and recruitedheterogenic stromal components. The study of the interactions between theperivascular niche and its surrounding cells is of great value in unravelingmechanisms of drug resistance in malignant gliomas.In this study, we isolated the stromal diploid cell population fromoligodendroglioma and a mixed population of tumor aneuploid and stromaldiploid cells from astrocytoma specimens. The stromal cells expressedneural stem/progenitor and mesenchymal markers showing the samediscordant phenotype that is typical for glioma cells. Moreover, some ofthe stromal cells expressed CD133. For the first time, we demonstratedthat this type of stromal cells had the typical myofibroblastic phenotypeas the α-SMA+ cells formed α-SMA fibers and exhibited the specificfunction to deposit extracellular matrix (ECM) proteins at least in vitro.Immunofluorescent analysis showed diffuse or focal α-SMA staining in thecytoplasm of the astrocytoma-derived, A172, T98G, and U251MG gliomacells. We could suggest that α-SMA may be one of the main molecules,bearing protective functions. Possible mechanisms and consequences ofα-SMA disruptions in gliomas are discussed.
- Добавил в систему: Толкунова Елена Николаевна