Mechanisms of Formation, Structure, and Dynamics of Lipoprotein Discs Stabilized by Amphiphilic Copolymers: A Comprehensive Review

Orekhov, P.S.; Bozdaganyan, M.E.; Voskoboynikova, N.; Mulkidjanian, A.Y.; Karlova, M.G.; Yudenko, A.; Remeeva, A.; Ryzhykau, Y.L.; Gushchin, I.; Gordeliy, V.I.; Sokolova, O.S.; Steinhoff, H.; Kirpichnikov, M.P.; Shaitan, K.V.

Авторы: Orekhov Philipp S., Bozdaganyan Marine E., Voskoboynikova Natalia, Mulkidjanian Armen Y., Karlova Maria G., Yudenko Anna, Remeeva Alina, Ryzhykau Yury L., Gushchin Ivan, Gordeliy Valentin I., Sokolova Olga S., Steinhoff Heinz-Jürgen, Kirpichnikov Mikhail P., Shaitan Konstantin V.
Журнал: Nanomaterials
Том: 12
Номер: 361
Год издания: 2022
Издательство: MDPI
Местоположение издательства: Basel, Switzerland
Первая страница: 1
Последняя страница: 27
Номер статьи: 361
DOI: 10.3390/nano12030361
Аннотация: Abstract: Amphiphilic copolymers consisting of alternating hydrophilic and hydrophobic unitsaccount for a major recent methodical breakthrough in the investigations of membrane proteins.Styrene–maleic acid (SMA), diisobutylene–maleic acid (DIBMA), and related copolymers have beenshown to extract membrane proteins directly from lipid membranes without the need for classicaldetergents. Within the particular experimental setup, they form disc-shaped nanoparticles with anarrow size distribution, which serve as a suitable platform for diverse kinds of spectroscopy andother biophysical techniques that require relatively small, homogeneous, water-soluble particles ofseparate membrane proteins in their native lipid environment. In recent years, copolymer-encasednanolipoparticles have been proven as suitable protein carriers for various structural biology applications,including cryo-electron microscopy (cryo-EM), small-angle scattering, and conventional andsingle-molecule X-ray diffraction experiments. Here, we review the current understanding of howsuch nanolipoparticles are formed and organized at the molecular level with an emphasis on theirchemical diversity and factors affecting their size and solubilization efficiency.Keywords: SMA; DIBMA; amphiphilic copolymers; lipodiscs; nanolipoparticles; structural biology
Добавил в систему: Шайтан Константин Вольдемарович

	ИСТИНА	Войти в систему Регистрация
	ФНКЦ РР
	Главная Поиск Статистика О проекте Помощь

ИСТИНА

ФНКЦ РР

Mechanisms of Formation, Structure, and Dynamics of Lipoprotein Discs Stabilized by Amphiphilic Copolymers: A Comprehensive Reviewстатья

Прикрепленные файлы