|
ИСТИНА |
Войти в систему Регистрация |
ФНКЦ РР |
||
Complex of HIV-1 Integrase with Cellular Ku Protein: Interaction Interface and Search for Inhibitorsстатья Исследовательская статья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 20 апреля 2022 г.
- Авторы: Ilgova Ekaterina, Galkin Simon, Khrenova Maria, Serebryakova Marina, Gottikh Marina, Anisenko Andrey
- Журнал: International Journal of Molecular Sciences
- Том: 23
- Год издания: 2022
- Издательство: MDPI
- Местоположение издательства: Basel, Switzerland
- Первая страница: 2908
- DOI: 10.3390/ijms23062908
- Аннотация: The interaction of HIV-1 integrase and the cellular Ku70 protein is necessary for HIVreplication due to its positive effect on post-integration DNA repair. We have previously described in detail the Ku70 binding site within integrase. However, the integrase binding site in Ku70 remained poorly characterized. Here, using a peptide fishing assay and site-directed mutagenesis, we have identified residues I72, S73, and I76 of Ku70 as key for integrase binding. The molecular dynamics studies have revealed a possible way for IN to bind to Ku70, which is consistent with experimental data. According to this model, residues I72 and I76 of Ku70 form a “leucine zipper” with integrase residues, and, therefore, their concealment by low-molecular-weight compounds should impede the Ku70 interaction with integrase. We have identified such compounds by molecular docking and have confirmed their capacity to inhibit the formation of the integrase complex with Ku70. Our data demonstrate that the site of IN binding within Ku70 identified in the present work may be used for further search for inhibitors of the integrase binding to Ku70.
- Добавил в систему: Готтих Марина Борисовна
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | Int_J_Mol_Sci-2022.pdf | Int_J_Mol_Sci-2022.pdf | 1,5 МБ | 9 марта 2022 [gottikh] |