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Effect of the Reactivating Factor of Luteococcus japonicus subsp. casei on the Expression of SOS Response Genesстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 14 сентября 2013 г.
- Авторы: Loiko N.G., Vorob’eva L.I., Khodzhaev E.Yu, Kozlova A.N., Gal’chenko V.F., El’-Registan G.I.
- Журнал: Microbiology
- Том: 82
- Номер: 2
- Год издания: 2013
- Издательство: Pleiades Publishing, Ltd
- Местоположение издательства: Road Town, United Kingdom
- Первая страница: 126
- Последняя страница: 132
- DOI: 10.1134/S0026261713020094
- Аннотация: Abstract—The effect of the extracellular peptide reactivating factor (RF) synthesized by Luteococcus casei on stress response of Escherichia coli cells subjected to UV irradiation was studied. For these studies, we con- structed a test strain carrying the umuD–lacZ operon. The expression rate of this operon reflects the rate of SOS response. Protective effect of RF, defined as the number of cells retaining the colony-forming activity (CFU) after UV irradiation (49–1166 J/m2), was dose-dependent, species-nonspecific, and increasing with increase of the stress load. RF was demonstrated to possess the properties of a direct adaptogen: 15 min of preincubation with RF caused a 1.5–6-fold decrease in expression of the umuD SOS response gene in UV-treated cells, con- currently with a 1.2–7.5 times increase in the number of viable cells (those having retained their colony-forming activity). The probable mechanisms of the protective effect of RF are being discussed. Keywords: reactivating factor (RF), Luteococcus japonicus subsp. casei, stress, protection, UV irradiation, E.coli, SOS response, umuD–lacZ hybrid operon
- Добавил в систему: Ходжаев Евгений Юрьевич