The Number and Morphological Diversity of Bacteriophages in Soilsстатья
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Аннотация:Epifluorescence microscopy revealed the presence of bacteriophages in all samples of the studiedsoils (Haplic Chernozem, Albic Retisol (Cutanic, Siltic), Haplic Kastanozem, Fluvisol (Loamic, Humic)),Limnic Fluvisol, Dystric Fibric Histosol, Histic Leptosol, and Arenosol). The number of bacteriophagescounted using the SYBR Green I dye was significant: 0.34–5.7 billion units/g soil. Transmission electronmicroscopy was used to determine the icosahedral, caudate, and filamentous morphotypes of bacteriophages.Comparison of the amount of bacteriophages and bacteria showed that the number of phages in thestudied soil samples was slightly lower as compared to bacteria (except for the samples from Chernozem andfrom the Antarctic Arenosol, where the number of phages slightly exceeded that of bacteria). The ratiobetween the numbers of bacteriophages and bacteria varied from 0.16 to 1.67. The number of cells of gramnegativeand gram-positive bacteria was determined using the L7005 dye and compared with the number ofbacteriophages in samples of Histosol and Kastanozem. The predomination of gram-negative bacteria(61% of the total number of bacteria) was recorded. The number of phages in the waterlogged biotope (Histosol)was three times higher than in the dry biotope (Kastanozem). The revealed high abundance and significantmorphological diversity of phages enable us to suggest their particular role in the regulation of the numberof bacteria in soils.Keywords: soils, SYBR Green 1, number of bacteria, transmission electron microscopy, WRBDOI: 10.1134/S1064229322030048