|
ИСТИНА |
Войти в систему Регистрация |
ФНКЦ РР |
||
Phytomix-40 prevents developmen of experimental parkinsonian syndromeтезисы доклада
- Авторы: Bocharov E.V., Kucheryanu V.G., Bocharova O.A., Karpova R.V.
- Сборник: Russian Journal of Nematology
- Том: 11
- Тезисы
- Год издания: 2004
- Место издания: Russian Society of Nematologists Москва, Россия
- Первая страница: 260
- Аннотация: Parkinsonian syndrome (PS) is one of the most spreading aging desease. It is considered to be due to the result of nigral dopaminergic neurons loss and damage leading to reduction of dopamine (DA) level in striatum. It was demonstrated that lipid peroxydation (LPO) intensification contributes to nigral cell damage in parkinsonism. Plant adaptogenes (biological response modifiers) were studied to have antiaging action. They increased the dopamine level in brain. It was also found their antioxydant, immunomodifying, antineoplastic, hypoglycemic, hypolipidemic activities. The purpose of this study was to evaluate the effect of phytomixture (Phm-40) consisted of the forty plant water-ethanol extracts (Rhodiola r. L.; Eleutherococcus s.; etc.) on the expression of parkinsonian symptoms, level of DA, its methabolites and LPO products in the striatum of mice C57BL/6 with experimental PS.Methods: PS was induced by systemic administration of neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydro-pyridine (MPTP, 20 mg/kg, daily, i.p., every 12 h, during 10 days) in old mice C57BL/6. The development of PS was estimated by muscle rigidity and motor activity, which was measured in automated test system Opto-Varimex-3 ("Columbus Instruments", USA). PhM-40 was administrered to mice per os with drinking water in 10%-concentration 5 days before and 10 days following the beginning of MPTP injection. Using HPLC/ED we analysed DA, 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanilic acid (HVA) in mice striatum ex vivo on the day 11 after the beginning of MPTP injection. The level of LPO products, malon dialdehyde (MDA), in the mice striatum was measured by the spectrofluorimetric method.Results: The administration of PhM-40 resulted in an increase of animal motor and other forms of locomotor activity and reduced rigidity on day 5 and day 10 after the beginning of MPTP injection. MPTP administration caused essential reduction of DA, HVA and DOPAC levels in the mice striatum. Chronic administration of PhM-40 in parkinsonian mice prevented LPO products formation and increased the striatal levels of DA, DOPAC and HVA by 46%, in 4.1 and 2.3 times, correspondingly.Conclusion: The results demonstrate that long-term administration of PhM-40 with drinking water in PS mice prevents parkinsonian symptoms formation, partially restored DA and its metabolites deficits and blocked LPO products in the striatum. It is suggested that PhM-40 possess neuroprotective properties that explains it antiparkinsonian effect in mice.
- Добавил в систему: Бочарова Ольга Алексеевна