Identification of the telomerase RNA in thermotolerant yeast Hansenula polymorphaстатья
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Аннотация:Telomerase is a ribonucleoprotein which maintains eukaryotic genome integrity via replication of the chromosomal ends. The core enzyme comprises two main components – protein TERT (telomerase reverse transcriptase) which is conserved among all the species and RNA subunit, which in contrary varies a lot in size and sequence which makes it particularly difficult to identify the one from the novel organism. Here we report identification of the telomerase RNA from thermotolerant yeast H. polymorpha. It is one of the smallest among yeast species and consists of 824 nt. Mutational analysis of its template and investigation of the H. polymorpha telomeres revealed that the mechanism of the H. polymorpha telomerase action differs significantly in vivo and in vitro. Telomerase, isolated in vitro efficiently incorporates to the newly synthesized repeat additional noncognate nucleotide which is not present in telomeres. We propose that this action corresponds to nonprocessive state of telomerase and reflects regulation between active and inactive form of the enzyme. This work was supported by: Russian Ministry of Education and Science (P800, P1390 No 02.740.11.07.06, No 16.512.11.2108) and Russian Foundation for Basic Research (Grants 11-04-01310-a and 11-04-12051-ofi-m-2011; PNR 5.13).