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Increased Extracellular Sodium Concentration as a Factor Regulating Gene Expression in Endotheliumстатья
Информация о цитировании статьи получена из
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 20 июля 2022 г.
- Авторы: Fedorov Dmitry A., Sidorenko Svetlana V., Yusipovich Alexander I., Bukach Olesya V., Gorbunov Andrey M., Lopina Olga D., Klimanova Elizaveta A.
- Журнал: Biochemistry (Moscow)
- Том: 87
- Номер: 6
- Год издания: 2022
- Издательство: Pleiades Publishing, Ltd
- Местоположение издательства: Road Town, United Kingdom
- Первая страница: 489
- Последняя страница: 499
- DOI: 10.1134/S0006297922060013
- Аннотация: Hyperosmotic stimulation of endothelial cells often leads to its dysfunction accompanied, among other things, byproinfl ammatory response. The mechanisms of this phenomenon are not fully understood. It may arise due to increase in the plasma Na+ concentration, due to increase in the extracellular osmolarity, increase in the intracellular Na+i/K+i ratio, and/or change in the cell stiff ness. In the present study we investigated the eff ects of short-term increase in osmolarity of extracellular medium on the mRNA content of some genes important for endothelial function (including Na+i/K+i-sensitive ones) and the equivalent elasticity constant of human umbilical vein endothelial cells membranes. Hyperosmotic stimulation of these cells with NaCl but not mannitol resulted in accumulation of Na+ ions inside the cells despite the Na,K-ATPase activation, and was also accompanied by the decrease in their equivalent elasticity constant. The amount of IL1α mRNA decreased with increasing osmolarity of the extracellular medium, whereas the amount of ATF3, PAR2, and PTGS2 mRNAs increased only in response to the increasing NaCl concentration. At the same time, under the conditions of our experiments, we did not detect changes in the expression of the osmoprotective transcription factor NFAT5. The obtained data indicate that the increase of extracellular Na+ concentration in the physiological range is an independent factor that aff ects intracellular Na+i/K+i ratio andregulates expression of some genes (in particular, ATF3, PAR2, PTGS2) in endothelial cells.
- Добавил в систему: Сидоренко Светлана Вадимовна