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A Novel Cre/lox71-Based System for Inducible Expression of Recombinant Proteins and Genome Editingстатья Электронная публикация
Информация о цитировании статьи получена из
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 20 июля 2022 г.
- Авторы: Karagyaur Maxim, Dyikanov Daniyar, Tyurin-Kuzmin Pyotr, Dzhauari Stalik, Skryabina Mariya, Vigovskiy Maksim, Primak Alexandra, Kalinina Natalia, Tkachuk Vsevolod
- Журнал: Cells
- Том: 11
- Номер: 14
- Год издания: 2022
- Издательство: MDPI
- Местоположение издательства: Basel, Switzerland
- Номер статьи: 2141
- DOI: 10.3390/cells11142141
- Аннотация: In this study, we developed a novel Cre/lox71-based system for the controlled transient expression of target genes. We used the bacteriophage P1 Cre recombinase, which harbors a short, highly specific DNA-binding site and does not have endogenous binding sites within mouse or human genomes. Fusing the catalytically inactive form of Cre recombinase and the VP64 transactivation domain (VP16 tetramer), we constructed the artificial transcription factor Cre-VP64. This transcription factor binds to the lox71 sites within the promoter region of the target gene and, therefore, upregulates its expression. We tested the Cre-VP64/lox71 system for the controlled expression of several genes, including growth factors and the genome editor CRISPR/Cas9, and obtained superior efficiency in the regulation of transgene expression, achieving a high expression level upon induction together with low basal activity. This system or its modified forms can be suggested as a novel effective tool for the transitory controlled expression of target genes for functional genomic studies, as well as for gene therapy approaches.
- Добавил в систему: Карагяур Максим Николаевич