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Degradation of mycotoxins in mixtures by combined proteinous nanobiocatalysts: In silico, in vitro and in vivoстатья Исследовательская статья
Статья опубликована в высокорейтинговом журнале
Статья опубликована в журнале из списка Web of Science и/или Scopus- Авторы: Lyagin Ilya, Maslova Olga, Stepanov Nikolay, Efremenko Elena
- Журнал: International Journal of Biological Macromolecules
- Том: 218
- Год издания: 2022
- Издательство: Elsevier BV
- Местоположение издательства: Netherlands
- Первая страница: 866
- Последняя страница: 877
- DOI: 10.1016/j.ijbiomac.2022.07.179
- Аннотация: New combined proteinous (enzymatic) nanobiocatalysts capable of destructing mycotoxins in mixtures were developed and investigated in vitro and in vivo. Candidate enzymes for such combined biocatalysts were computationally screened using molecular docking of mycotoxins to the proteins. Catalytic characteristics of the 7 selected enzymes were estimated in the potential reactions with various mycotoxins (aflatoxin B1, citrinin, deoxynivalenol, ergotamine, fumonisin B1, gliotoxin, ochratoxin A, patulin, sterigmatocystin, T-2 toxin, zearalenone) at different pH values. To stabilize the enzymes hydrolyzing the mycotoxins, special biopolymers were selected using computer modeling. The poly(glutamic acid) was revealed as universal partner for the polyelectrolyte complexes with the selected enzymes. Finally, Sprague-Dawley rats were used for in vivo feeding experiments with feed contaminated by mycotoxin mixture at doses being up to orders of magnitude higher than maximum allowable limits. The treatment of contaminated feed by novel combined enzyme nanocomplexes significantly decreased negative effects of mycotoxin mixture on blood biochemical parameters which indicated huge damage to liver and kidneys of intoxicated animals. Such nanobiocatalysts and enzymatic treatment itself seem to be promising way for ensuring both food and feed chemical safety.https://authors.elsevier.com/c/1fV8RWFfgpK9r
- Добавил в систему: Лягин Илья Владимирович
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