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Structure of an Intranucleosomal DNA Loop That Senses DNA Damage during Transcriptionстатья
- Авторы: Gerasimova Nadezhda S., Volokh Olesya I., Pestov Nikolay A., Armeev Grigory A., Kirpichnikov Mikhail P., Shaytan Alexey K., Sokolova Olga S., Studitsky Vasily M.
- Журнал: Cells
- Том: 11
- Номер: 17
- Год издания: 2022
- Издательство: MDPI
- Местоположение издательства: Basel, Switzerland
- Первая страница: 2678
- DOI: 10.3390/cells11172678
- Аннотация: Transcription through chromatin by RNA polymerase II (Pol II) is accompanied by the formation of small intranucleosomal DNA loops containing the enzyme (i-loops) that are involved in survival of core histones on the DNA and arrest of Pol II during the transcription of damaged DNA. However, the structures of i-loops have not been determined. Here, the structures of the intermediates formed during transcription through a nucleosome containing intact or damaged DNA were studied using biochemical approaches and electron microscopy. After RNA polymerase reaches position +24 from the nucleosomal boundary, the enzyme can backtrack to position +20, where DNA behind the enzyme recoils on the surface of the histone octamer, forming an i-loop that locks Pol II in the arrested state. Since the i-loop is formed more efficiently in the presence of SSBs positioned behind the transcribing enzyme, the loop could play a role in the transcription-coupled repair of DNA damage hidden in the chromatin structure.
- Добавил в систему: Герасимова Надежда Сергеевна