|
ИСТИНА |
Войти в систему Регистрация |
ФНКЦ РР |
||
DispHScan: A Multi-Sequence Web Tool for Predicting Protein Disorder as a Function of pHстатья
- Авторы: Pintado-Grima C., Iglesias V., Santos J., Uversky V.N., Ventura S.
- Журнал: Biomolecules
- Том: 11
- Номер: 11
- Год издания: 2021
- Издательство: MDPI
- Местоположение издательства: Basel, Switzerland
- Аннотация: Proteins are exposed to fluctuating environmental conditions in their cellular context and during their biotechnological production. Disordered regions are susceptible to these fluctuations and may experience solvent-dependent conformational switches that affect their local dynamism and activity. In a recent study, we modeled the influence of pH in the conformational state of IDPs by exploiting a charge-hydrophobicity diagram that considered the effect of solution pH on both variables. However, it was not possible to predict context-dependent transitions for multiple sequences, precluding proteome-wide analysis or the screening of collections of mutants. In this article, we present DispHScan, the first computational tool dedicated to predicting pH-induced disorder-order transitions in large protein datasets. The DispHScan web server allows the users to run pH-dependent disorder predictions of multiple sequences and identify context-dependent conformational transitions. It might provide new insights on the role of pH-modulated conditional disorder in the physiology and pathology of different organisms. The DispHScan web server is freely available for academic users, it is platform-independent and does not require previous registration.
- Добавил в систему: Уверский Владимир Николаевич