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Detection of kanamycin and gentamicin residues in animal-derived food using IgY antibody based ic-ELISA and FPIAстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 25 апреля 2017 г.
- Авторы: Cui Li, Zhang Yaoyao, Eremin Sergei A., Yakup Omar, Yao Gang, Zhang Xiaoying
- Журнал: Food Chemistry
- Том: 227
- Год издания: 2017
- Издательство: Elsevier BV
- Местоположение издательства: Netherlands
- Первая страница: 48
- Последняя страница: 54
- DOI: 10.1016/j.foodchem.2017.01.058
- Аннотация: Our aim in this study is to show that IgY antibody based immunoassays could be used to detect antibiotic residues in animal-derived food. Briefly, full antigens of gentamicin (Gent) and kanamycin (Kana) were used to immunize the laying chickens to prepare IgY antibodies. Then, these antibodies were evaluated by FPIA and ic-ELISA to detect Gent/Kana in animal-derived samples. The IC50 of FPIA and ic-ELISA based anti-Gent IgY were 7.70 ± 0.6 ug/mL and 0.32 ± 0.06 ug/mL, respectively. The IC50 of FPIA and ic-ELISA based anti-Kana IgY were 7.97 ± 0.9 ug/mL and 0.15 ± 0.01 ug/mL. The limits of detection (LOD, IC10) for FPIA based anti-Gent/Kana IgY were 0.17 and 0.007 lg/mL, respectively. The LOD for ic-ELISA were both 0.001 lg/mL. These results indicated that the ic-ELISA might more suitable for antibiotic residues detection than FPIA.
- Добавил в систему: Еремин Сергей Александрович
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | 17_FoodChem227_48_KAN_GEN_IgY.pdf | 17_FoodChem227_48_KAN_GEN_IgY.pdf | 935,9 КБ | 20 марта 2017 [Sergei_A_EREMIN] |