Bacteriolytic enzymes from the blood plasma of the sheepстатья
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Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
Аннотация:Bacteriolytic factors from the blood plasma of healthy sheep have been studied. Three enzymes not described earlier in the literature have been identified and characterized. Two enzymes exhibit activity toward Escherichia coli and have molecular weights of 15 ± 2 kDa. The third enzyme that exhibits activity toward E. coli and Micrococcus luteus has a molecular weight of 34 ± 4 kDa. The kinetic parameters of bacterial lysis for all enzymes have been determined; in particular, optimal pH values for each of the substrates used have been found. For the identification of the enzymes, trypsinolysis and a massspectroscopic study of fragments have been carried out. The results were compared with the data on sheep proteins available in the
SwissProt, NCBI, and MSDB databases. Keywords: bacteriolytic enzyme, blood plasma, Escherichia coli, Micrococcus luteus, Bacillus subtilis