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Белки опухолевых клеток, ыявляемые с помощью антител к С- и Т- концевым фрагментам продукта гена FOSстатья
Статья опубликована в журнале из списка RSCI Web of Science
Статья опубликована в журнале из перечня ВАКСтатья опубликована в журнале из списка Web of Science и/или Scopus
- Авторы: Мазуренко Н.Н., Киселева Н.П., Кудрявец Ю.И., Михалева И.И., Антоненко В.В., Киселев Ф.Л.
- Журнал: Молекулярная биология
- Том: 24
- Номер: 6
- Год издания: 1990
- Первая страница: 1351-
- Последняя страница: 1362
- Аннотация: Mol Biol (Mosk) . 1990 Sep-Oct;24(5):1351-62. [Tumor cell proteins, detected using antibodies to the S- and N-terminal fragments of the fos proto-oncogene product] [Article in Russian] N N Mazurenko, N P Kiseleva, Iu I Kudriavets, I I Mikhaleva, V V Antonenko, F L Kiselev • PMID: 2127073 Abstract Antibodies to c-fos oncoprotein were produced in rabbits by immunization with synthetic peptides, corresponding to the sequences 6-15 of N-end and 371-380 of C-end of c-fos oncoprotein. C-fos expression was tested with immunoprecipitation and immunoblotting in various transformed cell lines with antibodies to N- and C-decapeptides. It was shown that antibodies to C-terminal decapeptide revealed a c-fos gene product and also some fos-related antigens FRAs 36 kD, 46 kD, 75 kD and 90 kD in rat pheochromocytoma PC-12 cells and mouse carcinoma cell lines MAC-3 and LL. In some cell lines 46 kD FRA was expressed in the absence of p62 c-fos. Besides, different clones of the same cell line cultivated in identical conditions revealed differences in the 46 kD FRA expression. Antibodies to sequence 6-15 of N-end revealed only c-fos products and no FRAs were detected. Therefore FRAs have homology with the c-fos product in the C-terminal region and differ from it in the N-terminal region.
- Добавил в систему: Мазуренко Наталья Николаевна