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Gamma globulins-induced interaction between two red blood cells: forces measurement with optical tweezersстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Дата последнего поиска статьи во внешних источниках: 14 августа 2017 г.
- Авторы: Kisung Lee, Muravyov Alexei, Semenov Alexey, Wagner Christian, Priezzhev Alexander, Lyubin Eugeny, Fedyanin Andrey
- Сборник: Proceedings of SPIE
- Серия: Proceedings of Saratov Fall Meeting 2016: Optical Technologies in Biophysics & Medicine XVII
- Том: 10336
- Год издания: 2017
- Издательство: SPIE
- Местоположение издательства: Bellingham, WA, United States
- Первая страница: 10336056
- DOI: 10.1117/12.2271646
- Аннотация: The protein contribution to the red blood cell (RBC) aggregation is studied using the in-house made two-channeled optical tweezers. The cells interaction was characterized using two forces: the force required for separating two cells (FD – disaggregating force) and the force required for holding them from their spontaneous aggregation (FA – aggregating force). The gamma globulin solutions with/without albumin were used to induce the RBC aggregation. The strong interaction (3-10 pN) between the cells was measured within the contact formed using optical tweezers. We found that FD becomes stronger as the gamma globulin concentration increases, while the addition of albumin to the solution led to the significant (few fold) enhancement of the cells interaction forces. However, despite of the strong interaction between the cells their spontaneous overlapping was not observed, unlike the case in plasma, where the cells did increase their overlapping surface, when attached with small interacting surface and released from optical traps. This work in addition to our previous work with model solutions of fibrinogen allows us to conclude that the synergy of blood components is one of the most important features that contribute to the reversible RBC aggregation.
- Добавил в систему: Семенов Алексей Николаевич