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Colocalization analysis of cytoplasmic actin isoforms distribution in endothelial cellsстатья
Статья опубликована в высокорейтинговом журнале
Статья опубликована в журнале из списка Web of Science и/или Scopus- Авторы: Shakhov A.S., Kovaleva P.A., Churkina A.S., Kireev I.I., Alieva I.B.
- Журнал: BIOMEDICINES
- Том: 10
- Номер: 12
- Год издания: 2022
- Первая страница: 1
- Последняя страница: 14
- Номер статьи: biomedicines-1975665
- DOI: 10.3390/biomedicines10123194
- Аннотация: Actin cytoskeleton is an essential component of living cells and plays a decisive role in many cellular processes. In mammals, β- and γ-actin are cytoplasmic actin isoforms in non-muscle cells. Despite minor differences in the amino acid sequence, β- and γ-actin localize in different cell structures and perform different functions. While cytoplasmic β-actin is involved in many intracellular processes including cell contraction, γ-actin is responsible for cell mobility and promotes tumor transformation. Numerous studies demonstrate that β- and γ-actin are spatially separated in the cytoplasm of fibroblasts and epithelial cells; this separation is functionally determined. The spatial location of β/γ-actin in endothelial cells is still a subject for discussion. Using super-resolution microscopy, we investigated the β/γ-actin colocalization in endotheliocytes and showed that the β/γ-actin colocalization degree varies widely between different parts of the marginal regions and near the cell nucleus. In the basal cytoplasm, β-actin predominates, while the ratio of isoforms evens out as it moves to the apical cytoplasm. Thus, our colocalization analysis suggests that β- and γ-actin are segregated in the endotheliocyte cytoplasm. The segregation is greatly enhanced during cell lamella activation in the nocodazole-induced endothelial barrier dysfunction reflecting a different functional role of cytoplasmic actin isoforms in endothelial cells. Key words: endothelial cell, actin cytoskeleton, non-muscle actin isoforms, β-actin, γ-actin, super resolution microscopy, colocalization analysis
- Добавил в систему: Алиева Ирина Борисовна