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Comparison of the individual and combined actions of charged amino acids and glycine on the lysis of Escherichia coli cells by human and chicken lysozymeстатья
- Авторы: Rastriga Nikolay V., Klimov Dmitry A., Gasanova Dariya A., Levashov Pavel A.
- Журнал: Process Biochemistry
- Том: 125
- Год издания: 2023
- Издательство: Elsevier Applied Science
- Местоположение издательства: United Kingdom
- Первая страница: 190
- Последняя страница: 197
- DOI: 10.1016/j.procbio.2022.12.024
- Аннотация: The current study focussed on the regulation of the bacteriolytic activity of lysozyme with regard to the bacterial cells of Escherichia coli and the influence on it by the presence of various combinations of charged amino acids and glycine. The effects of combinations of the aforementioned amino acids on the activity of lysozyme had not been previously investigated. The effect of enhancing enzyme antibacterial activity in the presence of effectors was compared in the lysozymes of humans (HL) and those of chickens (CL), taking into account the influence of ionic strength and pH. It was discovered that the use of double and triple combinations of amino acids could lead to significantly greater activation of lysozyme than was previously possible using single effectors. A particularly significant increase in bacteriolytic activity was observed when using a buffer solution with low ionic strength (5–10 mM). An increase in lysozyme activity of approximately 10 times was attained under the simultaneous presence of glutamate, histidine, and arginine for the human enzyme and with the simultaneous presence of lysine, histidine, and arginine for that found in chickens (5 mM of each amino acid).
- Добавил в систему: Левашов Павел Андреевич