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Mechanistic comparison of Bacillus subtilis 6S-1 and 6S-2 RNAs - commonalities and differencesстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 2 июня 2014 г.
- Авторы: Burenina O.Y., Hoch P.G., Damm K., Salas M., Zatsepin T.S., Lechner M., Oretskaya T.S., Kubareva E.A., Hartmann R.K.
- Журнал: RNA
- Том: 20
- Номер: 3
- Год издания: 2014
- Первая страница: 348
- Последняя страница: 359
- DOI: 10.1261/rna.042077.113
- Аннотация: Bacterial 6S RNAs bind to the housekeeping RNA polymerase (sigA-RNAP in Bacillus subtilis) to regulate transcription in a growth phase-dependent manner. B. subtilis expresses two 6S RNAs, 6S-1 and 6S-2 RNA, with different expression profiles. We show in vitro that 6S-2 RNA shares hallmark features with 6S-1 RNA: (i) both are able to serve as templates for pRNA transcription, (ii) bind with comparable affinity to sigA-RNAP, (iii) are able to specifically inhibit transcription from DNA promoters, and (iv) can form stable 6S RNA:pRNA hybrid structures that (v) abolish binding to sigA-RNAP. However, pRNAs of equal length dissociate faster from 6S-2 than 6S-1 RNA, owing to the higher A,U-content of 6S-2 pRNAs. This could have two mechanistic implications: (i) short 6S-2 pRNAs (< 10 nt) dissociate faster instead of being elongated to longer pRNAs, which should make it more difficult for 6S-2 RNA-stalled RNAP molecules to escape from an idling cycle of abortive pRNA transcription. (ii) Relative to 6S-1 RNA, 6S-2 pRNAs of equal length will dissociate more rapidly form 6S-1 RNA after RNAP release, which could affect pRNA turnover or the kinetics of 6S-2 RNA binding to a new RNAP molecule. With respect to the possibility that RNAP might be released from 6S-2 RNA sequestration by other means than pRNA synthesis in vivo, e.g. via direct displacement of 6S-2 RNA by 6S-1 RNA, this was analyzed in binding competition experiments, including conditions when synthesis of very short pRNAs (~5-mers) could take place.
- Добавил в систему: Буренина Ольга Юрьевна
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