Stability and Thrombogenicity Analysis of Collagen/CarbonNanotube Nanocomposite Coatings Using a ReversibleMicrofluidic Device

Popovich, K.D.; Vagner, S.A.; Murashko, D.T.; Ten Galina, N.; Ryabkin, D.I.; Savelyev, M.S.; Kitsyuk, E.P.; Gerasimenko, E.A.; Edelbekova, P.; Konovalov, A.N.; Telyshev, D.V.; Selishchev, S.V.; Gerasimenko, A.Y.

Авторы: Popovich Kristina D., Vagner Sergey A., Murashko Denis T., Ten Galina N., Ryabkin Dmitry I., Savelyev Mikhail S., Kitsyuk Evgeny P., Gerasimenko Ekaterina A., Edelbekova Polina, Konovalov Anton N., Telyshev Dmitry V., Selishchev Sergey V., Gerasimenko Alexander Yu
Журнал: Membranes
Номер: 13
Год издания: 2023
Издательство: MDPI Publishing
Местоположение издательства: Basel, Switzerland, Switzerland
DOI: 10.3390/membranes13040403
Аннотация: Abstract: Currently, the development of stable and antithrombogenic coatings for cardiovascularimplants is socially important. This is especially important for coatings exposed to high shear stressfrom flowing blood, such as those on ventricular assist devices. A method of layer-by-layer formationof nanocomposite coatings based on multi-walled carbon nanotubes (MWCNT) in a collagenmatrix is proposed. A reversible microfluidic device with a wide range of flow shear stresses hasbeen developed for hemodynamic experiments. The dependence of the resistance on the presenceof a cross-linking agent for collagen chains in the composition of the coating was demonstrated.Optical profilometry determined that collagen/c-MWCNT and collagen/c-MWCNT/glutaraldehydecoatings obtained sufficiently high resistance to high shear stress flow. However, the collagen/c-MWCNT/glutaraldehyde coating was almost twice as resistant to a phosphate-buffered solution flow.A reversible microfluidic device made it possible to assess the level of thrombogenicity of the coatingsby the level of blood albumin protein adhesion to the coatings. Raman spectroscopy demonstratedthat the adhesion of albumin to collagen/c-MWCNT and collagen/c-MWCNT/glutaraldehyde coatingsis 1.7 and 1.4 times lower than the adhesion of protein to a titanium surface, widely used forventricular assist devices. Scanning electron microscopy and energy dispersive spectroscopy determinedthat blood protein was least detected on the collagen/c-MWCNT coating, which containedno cross-linking agent, including in comparison with the titanium surface. Thus, a reversible microfluidicdevice is suitable for preliminary testing of the resistance and thrombogenicity of variouscoatings and membranes, and nanocomposite coatings based on collagen and c-MWCNT are suitablecandidates for the development of cardiovascular devices.
Добавил в систему: Коновалов Антон Николаевич

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Stability and Thrombogenicity Analysis of Collagen/CarbonNanotube Nanocomposite Coatings Using a ReversibleMicrofluidic Deviceстатья

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