Antiviral activity of singlet oxygen-photogenerating perylene compounds against SARS-CoV-2: Interaction with the viral envelope and photodynamic virion inactivation

Strakova, P.; Bednar, P.; Kotoucek, J.; Holoubek, J.; Fortova, A.; Svoboda, P.; Stefanik, M.; Huvarova, I.; Simeckova, P.; Masek, J.; Gvozdev, D.A.; Mikhnovets, I.E.; Chistov, A.A.; Nikitin, T.D.; Krasilnikov, M.S.; Ustinov, A.V.; Alferova, V.A.; Korshun, V.A.; Růzek, D.; Eyer, L.

Авторы: Strakova Petra, Bednar Petr, Kotoucek Jan, Holoubek Jirí, Fortova Andrea, Svoboda Pavel, Stefanik Michal, Huvarova Ivana, Simeckova Pavlína, Masek Josef, Gvozdev Daniil A., Mikhnovets Igor E., Chistov Alexey A., Nikitin Timofei D., Krasilnikov Maxim S., Ustinov Alexey V., Alferova Vera A., Korshun Vladimir A., Růzek Daniel, Eyer Ludek
Журнал: Virus Research
Том: 334
Год издания: 2023
Издательство: Elsevier BV
Местоположение издательства: Netherlands
Номер статьи: 199158
DOI: 10.1016/j.virusres.2023.199158
Аннотация: The coronavirus disease 2019 (COVID-19) pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has prompted great interest in novel broad-spectrum antivirals, including perylene-related compounds. In the present study, we performed a structure–activity relationship analysis of a series of perylene derivatives, which comprised a large planar perylene residue, and structurally divergent polar groups connected to the perylene core by a rigid ethynyl or thiophene linker. Most of the tested compounds did not exhibit significant cytotoxicity towards multiple cell types susceptible to SARS-CoV-2 infection, and did not change the expressions of cellular stress-related genes under normal light conditions. These compounds showed nanomolar or sub-micromolar dose-dependent anti-SARS-CoV-2 activity, and also suppressed the in vitro replication of feline coronavirus (FCoV), also termed feline infectious peritonitis virus (FIPV). Perylene compounds exhibited high affinity for liposomal and cellular membranes, and efficiently intercalated into the envelopes of SARS-CoV-2 virions, thereby blocking the viral–cell fusion machinery. Furthermore, the studied compounds were demonstrated to be potent photosensitizers, generating reactive oxygen species (ROS), and their anti-SARS-CoV-2 activities were considerably enhanced after irradiation with blue light. Our results indicated that photosensitization is the major mechanism underlying the anti-SARS-CoV-2 activity of perylene derivatives, with these compounds completely losing their antiviral potency under red light. Overall, perylene-based compounds are broad-spectrum antivirals against multiple enveloped viruses, with antiviral action based on light-induced photochemical damage (ROS-mediated, likely singlet oxygen-mediated), causing impairment of viral membrane rheology.
Добавил в систему: Гвоздев Даниил Александрович

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Antiviral activity of singlet oxygen-photogenerating perylene compounds against SARS-CoV-2: Interaction with the viral envelope and photodynamic virion inactivationстатья

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