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A New Complex of the Ribozyme with Glucose-6-Phosphate Isomerase Activity and the Enzyme Hexokinase in Baker’s Yeast Saccharomyces cerevisiaeстатья Исследовательская статья
- Автор: Solovjeva O.N.
- Журнал: Russian Journal of Bioorganic Chemistry
- Том: 49
- Номер: 5
- Год издания: 2023
- Издательство: Maik Nauka/Interperiodica Publishing
- Местоположение издательства: Russian Federation
- Первая страница: 1014
- Последняя страница: 1022
- Оригинальные версии: Новый комплекс рибозима с глюкозо-6-фосфатизомеразной активностью и фермента гексокиназы в пекарских дрожжах Saccharomyces cerevisiae
- DOI: 10.31857/S013234232305007X
- Аннотация: The existence of a previously unknown ribozyme with the catalytic function of glucose-6-phosphate isomerase was shown. It catalyzes the interconversion of glucose 6-phosphate and fructose 6-phosphate. This ribozyme was found in baker’s yeast Saccharomyces cerevisiae and was isolated as a complex with the enzyme hexokinase. The complex was easily isolated on an immunoaffinity column with antibodies to hexokinase. The ribozyme consists of 41–42 nucleotides and has a molecular weight of about 14.15–14.5 kDa. Km and Vmax are accordingly 0.14 ± 0.02 mM and 14.0 ± 1.3 U/mg for glucose 6-phosphate and 0.2 ± 0.03 mM and 15.4 ± 1.4 U/mg for fructose 6-phosphate. These kinetic characteristics are approximately the same in the complex and for the free ribozyme. Hexokinase within the complex retains its catalytic activity.
- Добавил в систему: Соловьева Ольга Николаевна
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