High and Low Levels of ABCB1 Expression Are Associated with Two Distinct Gene Signatures in Lung Tissue of Pulmonary TB Patients with High Inflammation Activity

Pavlova, E.N.; Lepekha, L.N.; Rybalkina, E.Y.; Tarasov, R.V.; Sychevskaya, K.A.; Voronezhskaya, E.E.; Masyutin, A.G.; Ergeshov, A.E.; Erokhina, M.V.

Авторы: Pavlova Ekaterina N., Lepekha Larisa N., Rybalkina Ekaterina Yu, Tarasov Ruslan V., Sychevskaya Ksenia A., Voronezhskaya Elena E., Masyutin Alexander G., Ergeshov Atadzhan E., Erokhina Maria V.
Журнал: International Journal of Molecular Sciences
Том: 24
Номер: 19
Год издания: 2023
Издательство: MDPI
Местоположение издательства: Basel, Switzerland
DOI: 10.3390/ijms241914839
Аннотация: P-glycoprotein (encoded by the ABCB1 gene) has a dual role in regulating inflammation and reducing chemotherapy efficacy in various diseases, but there are few studies focused on pulmonary TB patients. In this study, our objective was to identify a list of genes that correlate with high and low levels of ABCB1 gene expression in the lungs of pulmonary TB patients with different activity of chronic granulomatous inflammation. We compared gene expression in two groups of samples (with moderate and high activity of tuberculomas) to identify their characteristic gene signatures. Gene expression levels were determined using quantitative PCR in samples of perifocal area of granulomas, which were obtained from 65 patients after surgical intervention. Subsequently, two distinct gene signatures associated with high inflammation activity were identified. The first signature demonstrated increased expression of HIF1a, TGM2, IL6, SOCS3, and STAT3, which correlated with high ABCB1 expression. The second signature was characterized by high expression of TNFa and CD163 and low expression of ABCB1. These results provide insight into various inflammatory mechanisms and association with P-gp gene expression in lung tissue of pulmonary TB patients and will be useful in the development of a host-directed therapy approach to improving the effectiveness of anti-TB treatment.
Добавил в систему: Масютин Александр Георгиевич

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High and Low Levels of ABCB1 Expression Are Associated with Two Distinct Gene Signatures in Lung Tissue of Pulmonary TB Patients with High Inflammation Activityстатья

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