Boric Acid Resistance in Drosophila melanogaster Depends on the Expression Level of the Cyp9b2 Geneстатья
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Дата последнего поиска статьи во внешних источниках: 15 февраля 2024 г.
Аннотация:Drosophila melanogaster is widely used as a model object in studies of molecular genetic mechanisms of response to effects of various xenobiotics, including insecticides. Boric acid is one of the most common insecticides, but the mechanisms of its effect on the insect organism have not been studied yet. Previously, the candidate gene Cyp9b2 was identified in D. melanogaster, the mutant for which acquires sensitivity to boric acid. In this work, we investigated the role of a cluster of two highly homologous genes Cyp9b1 and Cyp9b2 in the resistance of D. melanogaster to boric acid. The level of basic transcription of the Cyp9b2 gene is five times higher than that of the Cyp9b1 gene, which correlates with in silico analysis demonstrating the different regulatory potential of 5'-regulatory regions of these genes. Resistance to boric acid depends dose-specifically on the level of transcription of the Cyp9b2 gene, but not on the level of transcription of the Cyp9b1 gene. Inactivation of the Cyp9b1 gene does not affect the phenotype and does not affect the level of insecticide resistance.