C-Terminal Region of Caveolin-3 Contains a Stretch of Amino Acid Residues Capable of Diminishing Symptoms of Experimental Autoimmune Encephalomyelitis but Not Rheumatoid Arthritis Modeled in Rats

Danilkovich, A.V.; Turobov, V.I.; Palikov, V.A.; Palikova, Y.A.; Shepelyakovskaya, A.O.; Mikhaylov, E.S.; Slashcheva, G.A.; Shadrina, T.E.; Shaykhutdinova, E.R.; Rasskazova, E.A.; Tukhovskaya, E.A.; Khokhlova, O.N.; Dyachenko, I.A.; Ismailova, A.M.; Zinchenko, D.V.; Navolotskaya, E.V.; Lipkin, V.M.; Murashev, A.N.; Udovichenko, I.P.

Авторы: Danilkovich Alexey V., Turobov Valery I., Palikov Victor A., Palikova Yulia A., Shepelyakovskaya Anna O., Mikhaylov Evgeniy S., Slashcheva Gulsara A., Shadrina Tatiana E., Shaykhutdinova Elvira R., Rasskazova Ekaterina A., Tukhovskaya Elena A., Khokhlova Oksana N., Dyachenko Igor A., Ismailova Alina M., Zinchenko Dmitry V., Navolotskaya Elena V., Lipkin Valery M., Murashev Arkady N., Udovichenko Igor P.
Журнал: BIOMEDICINES
Том: 11
Номер: 10
Год издания: 2023
Первая страница: 2855
Последняя страница: 2875
DOI: 10.3390/biomedicines11102855
Аннотация: A short synthetic peptide from the C-terminal part of the caveolin-3 structure was tested for experimental autoimmune encephalomyelitis (EAE) treatment in rats. The structure–function similarity established between the novel synthetic peptide of pCav3 and the well-known immunomodulator immunocortin determined pCav3’s ability to reduce EAE symptoms in Dark Agouti (DA) rats injected with pCav3 (500 g/kg). pCav3 was found to interfere with the proliferation of lymphocytes extracted from the LNs of DA rats primed with homogenate injection, with IC50 = 0.42 M (2.35 mcg/mL). pCav3 affected EAE in a very similar manner as immunocortin. The high degree of homology between the amino acid sequences of pCav3 and immunocortin corresponded well with the therapeutic activities of both peptides, as demonstrated on EAE. The latter peptide, possessing a homologous structure to pCav3, was also tested on EAE to explore whether there were structural restrictions between these peptides implied by the MHC-involved cell machinery. Consequently, immunocortin was further examined with a different autoimmune disease model, collagen-induced arthritis (CIA), established in Sprague–Dawley rats. CIA was established using an intentionally different genetic platform than EAE. Based on the results, it was concluded that the effectiveness of pCav3 and immunocortin peptides in EAE rat model was almost identical, but differed in the rat model of rheumatoid arthritis; thus, efficacy may be sensitive to the MHC type of animals used to establish the autoimmune disease model.
Добавил в систему: Зинченко Дмитрий Валерьевич

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C-Terminal Region of Caveolin-3 Contains a Stretch of Amino Acid Residues Capable of Diminishing Symptoms of Experimental Autoimmune Encephalomyelitis but Not Rheumatoid Arthritis Modeled in Ratsстатья