Rate of elimination of human high-density lipoproteins after their intravenous administration in rabbits by the loss of alpha- cholesterol and apoprotein A-1статья
Дата последнего поиска статьи во внешних источниках: 23 ноября 2017 г.
Аннотация:Native HDL, HDL2 or HDL3, isolated from donor plasma were intravenously administered at a large dose (100 mg HDL cholesterol) to healthy rabbits and rabbits with experimental hypercholesterolemia. The elimination rate of human HDL was studied on the basis of alpha-cholesterol (alpha-Ch) and apoprotein (apo) A-I determinations in the plasma of rabbits. Administration of HDL or their subclasses at a large dose with the following determination of alpha-Ch or apo A-I enabled to avoid the use of radioactive label and all the methodological errors connected with the latter. After administration of HDL, HDL2 and HDL3 their elimination according to alpha-Ch determination occurred at the higher rate as compared with apo A-I. Elimination of the total HDL fraction according to alpha-Ch determination was higher in healthy rabbits than in animals with hypercholesterolemia while the elimination estimated by the apo A-I determination was similar in both groups of animals. These data suggest that HDL may characterize the Ch-donor properties. Apo A-I as compared with alpha-Ch was a more accurate indicator of HDL concentration in blood plasma.