Enhancing Human Glycoprotein Hormones Production in CHO Cells Using Heterologous Beta-Chain Signal Peptides

Авторы: Sinegubova M.V., Kolesov D.E., Dayanova L.K., Vorobiev I.I., Orlova N.A.
Журнал: Doklady Biochemistry and Biophysics
Год издания: 2023
Издательство: M A I K Nauka - Interperiodica
Местоположение издательства: Russian Federation
DOI: 10.1134/s1607672923700576
Аннотация: We studied the influence of heterologous signal peptides in the β-chains of glycoprotein hormoneson the biosynthesis of these hormones in a transiently transfected culture of Chinese hamster ovary cellsCHO S. When the natural signal peptides of the β-chains were replaced with the heterologous signal peptideof human serum albumin, cell productivity was increased 2–2.5 times for human luteinizing hormone,human chorionic gonadotropin, and human thyroid-stimulating hormone, but not for human follicle-stim-ulating hormone. No significant increase in cell productivity was observed for human azurocidin signal pep-tide and human glycoprotein hormone α-chain signal peptide. The used approach allows quick assessing theeffect of heterologous signal peptides on the biosynthesis of heterodimeric proteins of various classes.
Добавил в систему: Воробьев Иван Иванович

	ИСТИНА	Войти в систему Регистрация
	ФНКЦ РР
	Главная Поиск Статистика О проекте Помощь

ИСТИНА