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Design, optimization, characterization, and in vitro evaluation of metformin-loaded liposomes for triple negative breast cancer treatmentстатья
Информация о цитировании статьи получена из
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 17 апреля 2024 г.
- Авторы: Vozgirdaite Daiva, Hervé-Aubert Katel, Uzbekov Rustem, Chourpa Igor, Allard-Vannier Emilie
- Журнал: Journal of Liposome Research
- Том: 8
- Год издания: 2024
- Издательство: Marcel Dekker Inc.
- Местоположение издательства: United States
- Первая страница: 1
- Последняя страница: 15
- Аннотация: Recently, metformin (Met) has shown to have antineoplastic properties in cancer treatment by improv ing hypoxic tumor conditions, and causing reduction in the synthesis of biomolecules, which are vital for cancer growth. However, as an orally administered drug, Met has low bioavailability and rapid renal clearance. Thus, the goal of this study was to vectorize Met inside liposomes in the context of triple negative breast cancer (TNBC), which currently lacks treatment options when compared to other types of breast cancer. Vectorization of Met inside liposomes was done using Bangham method by imple menting double design of experiment methodology to increase Met drug loading (minimum-run reso lution V characterization design and Box-Behnken design), as it is generally extremely low for hydrophilic molecules. Optimization of Met-loaded liposome synthesis was successfully achieved with drug loading of 190mg/g (19% w/w). The optimal Met-liposomes were 170nm in diameter with low PdI (< 0.1) and negative surface charge (-20mV), exhibiting sustained Met release at pH 7.4. The lipo somal Met delivery system was stable over several months, and successfully reduced TNBC cell prolif eration due to the encapsulated drug. This study is one the first reports addressing liposome formulation through thin-film hydration using two design of experiment methods aiming to increase drug loading of Met
- Добавил в систему: Узбеков Рустем Эдуардович