Оn the aggregation of polycationic photosensitizer upon binding to Gram-negative bacteria

Romanishkin, I.D.; Akhlyustina, E.V.; Meerovich, G.A.; Loschenov, V.B.; Strakhovskaya, M.G.

Информация о цитировании статьи получена из Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 22 мая 2024 г.

Авторы: Romanishkin Igor D., Akhlyustina Ekaterina V., Meerovich Gennady A., Loschenov Victor B., Strakhovskaya Marina G.
Журнал: METHODS AND APPLICATIONS IN FLUORESCENCE
Том: 12
Номер: 3
Год издания: 2024
Первая страница: 035001
Последняя страница: 035001
DOI: 10.1088/2050-6120/ad3892
Аннотация: Polycationic photosensitizers (PS) are not susceptible to aggregation in solutions, but their high local concentrations in Gram-negative bacteria can be sufficient for aggregation and reduced effectiveness of antibacterial photodynamic treatment. By measuring fluorescence spectra and kinetics we were able to evaluate the degree of aggregation of polycationic PS ZnPcChol8 in Gram-negative bacteria E. coli K12 TG1. Binding of ZnPcChol8 to E. coli K12 TG1 leads to an appearance of groups of molecules with shorter PS fluorescence lifetime, a decrease in fluorescence intensity and a shift in the fluorescence spectral maximum. However, we evaluated that about 88% of the fluorescing PS molecules in the bacteria were in an unaggregated state, which indicates only a small reduction in the generation of reactive oxygen species.
Добавил в систему: Страховская Марина Глебовна

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Оn the aggregation of polycationic photosensitizer upon binding to Gram-negative bacteriaстатья

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