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Results of the early use of the amniotic membrane in an experimental model of corneal chemical burnстатья
- Авторы: Boyko E.V., Trunin E.M., Tatarkin V.V., Deev R.V., Kasymov F.O., Vasil’ev Yu L., Shchemerov N.V., Presnyakov E.V., Klitsenko O.A., Sorochanu I., Resnyanskaya E.D., Abdulminova R.I., Andreeva A.N.
- Журнал: Оперативная хирургия и клиническая анатомия (Пироговский научный журнал)
- Том: 7
- Номер: 4
- Год издания: 2023
- Первая страница: 15
- Последняя страница: 24
- DOI: 10.17116/operhirurg2023704115
- Аннотация: One of the important methods of treatment of chemical lesions of the cornea is the early covering of its surface with a flap of the amniotic membrane. Objective. Purpose of the work — to confirm the effectiveness of the developed system for histological assessment of the thickness of various layers of the cornea, to determine the advantages of early coverage of the eye surface with an amniotic membrane in combination with blepharorrhaphy and complex conservative therapy over a similar tactic without the use of AM in the experiment. Material and methods. The work was performed on Wistar rats. The animals were divided into 4 groups depending on the period of withdrawal from the experiment, the total number of animals was 32 (64 eyes). The corneal burn was formed using filter paper applicators impregnated with 1.0 molar NaOH solution. Then, before the eyes of the experimental group, the burn surface was washed for 10 minutes, followed by covering with a flap of the amniotic membrane and early blepharorrhaphy. In the eyes of the control group, a similar tactic was performed without covering the amniotic membrane. Subsequently, complex local conservative therapy was used for both groups. When animals were withdrawn from the experiment, such clinical signs as the safety of the blepharorrhaphy suture, reactive inflammation of the accessory organs of the eye, the state of the cornea (the degree of epithelialization, extensive neovascularization), subatrophy and tone of the eyeball were evaluated. Then, 2 rats were randomly selected from each group, in which the eyeballs were removed, followed by a histological assessment of the cut of the corneoscleral flap. The degree of pathological changes was assessed based on an objective measurement of the thickness of the corneal stroma and epithelium at multiple cut points, followed by a statistical evaluation of the data obtained. Results. In the amniotic membrane group, such clinical symptoms as reactive inflammation of the auxiliary organs of the eye, subatrophy of the eyeball were less often detected, and histological changes in the structure and thickness of the epithelium and stroma of the cornea were less pronounced. Conclusion. The results of the clinical and histological research confirm advantages of using native AM coating of the burn surface as an early treatment for alkaline chemical burns of the cornea
- Добавил в систему: Васильев Юрий Леонидович